Trypsin-Modified Human Erythrocytes
نویسندگان
چکیده
A method of treating human erythrocytes with trypsin has been modified and found to be an efficient and practical indicator system for the rubella hemagglutination-inhibition test. Both the trypsin-treated human cells and the widely used, newborn chicken erythrocytes were used in comparative testing of 464 selected diagnostic rubella serums. Results with each cell system were essentially the same. The trypsin treatment procedure has been found to be relatively simple, and with our limited testing has not presented any problems with reproducibility. Other advantages include the ready availability of human cells, greater intralaboratory standardization of the test by using the same donors over a long period of time, and elimination of adsorption of test sera with red blood cells.
منابع مشابه
Detection of rubella haemagglutination-inhibition (HAI) and virus-specific IgM antibody using trypsin-treated human group O erythrocytes in the HAI test.
The modification of the standard rubella haemagglutination-inhibition (HAI) test using trypsin-treated human group O erythrocytes instead of chick erythrocytes was evaluated. In a comparative study we found that, of 816 samples tested by both methods, the titres of 807 (98.9%) sera were in close agreement within an acceptable twofold difference. Trypsin-treated human group O erythrocytes usuall...
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